产品目录

保存：-20℃

载体基本信息
出品公司：Clontech
载体名称：pGADT7-T
质粒类型：酵母表达载体
克隆方法：多克隆位点，限制性内切酶
复制子：pUC
启动子：ADH1
载体大小：9972bp
5'测序引物及序列：CTATTCGATGATGAAGATACCCC
3'测序引物及序列：GTGAACTTGCGGGGTTTTTCAG
载体抗性：氨苄青霉素
筛选标记：LEU2
克隆菌株：DH5α等
宿主细胞(系)：酵母细胞
载体质粒图谱和多克隆位点信息
载体序列：
1 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
61 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
121 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
181 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
241 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
301 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
361 caccataacg catttaagca taaacacgca ctatgccgtt cttctcatgt atatatatat
421 acaggcaaca cgcagatata ggtgcgacgt gaacagtgag ctgtatgtgc gcagctcgcg
481 ttgcattttc ggaagcgctc gttttcggaa acgctttgaa gttcctattc cgaagttcct
541 attctctagc tagaaagtat aggaacttca gagcgctttt gaaaaccaaa agcgctctga
601 agacgcactt tcaaaaaacc aaaaacgcac cggactgtaa cgagctacta aaatattgcg
661 aataccgctt ccacaaacat tgctcaaaag tatctctttg ctatatatct ctgtgctata
721 tccctatata acctacccat ccacctttcg ctccttgaac ttgcatctaa actcgacctc
781 tacatttttt atgtttatct ctagtattac tctttagaca aaaaaattgt agtaagaact
841 attcatagag tgaatcgaaa acaatacgaa aatgtaaaca tttcctatac gtagtatata
901 gagacaaaat agaagaaacc gttcataatt ttctgaccaa tgaagaatca tcaacgctat
961 cactttctgt tcacaaagta tgcgcaatcc acatcggtat agaatataat cggggatgcc
1021 tttatcttga aaaaatgcac ccgcagcttc gctagtaatc agtaaacgcg ggaagtggag
1081 tcaggctttt tttatggaag agaaaataga caccaaagta gccttcttct aaccttaacg
1141 gacctacagt gcaaaaagtt atcaagagac tgcattatag agcgcacaaa ggagaaaaaa
1201 agtaatctaa gatgctttgt tagaaaaata gcgctctcgg gatgcatttt tgtagaacaa
1261 aaaagaagta tagattcttt gttggtaaaa tagcgctctc gcgttgcatt tctgttctgt
1321 aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttt
1381 tacaaaaatg aagcacagat tcttcgttgg taaaatagcg ctttcgcgtt gcatttctgt
1441 tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt
1501 tgttctacaa aatgaagcac agatgcttcg ttgcttgcat gcctgcaggt cgagatccgg
1561 gatcgaagaa atgatggtaa atgaaatagg aaatcaagga gcatgaaggc aaaagacaaa
1621 tataagggtc gaacgaaaaa taaagtgaaa agtgttgata tgatgtattt ggctttgcgg
1681 cgccgaaaaa acgagtttac gcaattgcac aatcatgctg actctgtggc ggacccgcgc
1741 tcttgccggc ccggcgataa cgctgggcgt gaggctgtgc ccggcggagt tttttgcgcc
1801 tgcattttcc aaggtttacc ctgcgctaag gggcgagatt ggagaagcaa taagaatgcc
1861 ggttggggtt gcgatgatga cgaccacgac aactggtgtc attatttaag ttgccgaaag
1921 aacctgagtg catttgcaac atgagtatac tagaagaatg agccaagact tgcgagacgc
1981 gagtttgccg gtggtgcgaa caatagagcg accatgacct tgaaggtgag acgcgcataa
2041 ccgctagagt actttgaaga ggaaacagca atagggttgc taccagtata aatagacagg
2101 tacatacaac actggaaatg gttgtctgtt tgagtacgct ttcaattcat ttgggtgtgc
2161 actttattat gttacaatat ggaagggaac tttacacttc tcctatgcac atatattaat
2221 taaagtccaa tgctagtaga gaaggggggt aacacccctc cgcgctcttt tccgattttt
2281 ttctaaaccg tggaatattt cggatatcct tttgttgttt ccgggtgtac aatatggact
2341 tcctcttttc tggcaaccaa acccatacat cgggattcct ataatacctt cgttggtctc
2401 cctaacatgt aggtggcgga ggggagatat acaatagaac agataccaga caagacataa
2461 tgggctaaac aagactacac caattacact gcctcattga tggtggtaca taacgaacta
2521 atactgtagc cctagacttg atagccatca tcatatcgaa gtttcactac cctttttcca
2581 tttgccatct attgaagtaa taataggcgc atgcaacttc ttttcttttt ttttcttttc
2641 tctctccccc gttgttgtct caccatatcc gcaatgacaa aaaaatgatg gaagacacta
2701 aaggaaaaaa ttaacgacaa agacagcacc aacagatgtc gttgttccag agctgatgag
2761 gggtatctcg aagcacacga aactttttcc ttccttcatt cacgcacact actctctaat
2821 gagcaacggt atacggcctt ccttccagtt acttgaattt gaaataaaaa aaagtttgct
2881 gtcttgctat caagtataaa tagacctgca attattaatc ttttgtttcc tcgtcattgt
2941 tctcgttccc tttcttcctt gtttcttttt ctgcacaata tttcaagcta taccaagcat
3001 acaatcaact ccaagctttg caaagatgga taaagcggaa ttaattcccg agcctccaaa
3061 aaagaagaga aaggtcgaat tgggtaccgc cgccaatttt aatcaaagtg ggaatattgc
3121 tgatagctca ttgtccttca ctttcactaa cagtagcaac ggtccgaacc tcataacaac
3181 tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc tctaacgttc atgataactt
3241 catgaataat gaaatcacgg ctagtaaaat tgatgatggt aataattcaa aaccactgtc
3301 acctggttgg acggaccaaa ctgcgtataa cgcgtttgga atcactacag ggatgtttaa
3361 taccactaca atggatgatg tatataacta tctattcgat gatgaagata ccccaccaaa
3421 cccaaaaaaa gagatcttta atacgactca ctatagggcg agcgccgcca tggagtaccc
3481 atacgacgta ccagattacg ctcatatggc catggaggcc agtgaattcg gaactgatga
3541 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
3601 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
3661 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
3721 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
3781 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
3841 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
3901 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
3961 tttgatgtat agtgccttga ctagagatcc attttctgtt attgaggaaa gtttgccagg
4021 tgggttaaag gagcatgatt ttaatccaga agaagcagag gaaactaaac aagtgtcctg
4081 gaagcttgta acagagtatg caatggaaac aaaatgtgat gatgtgttgt tattgcttgg
4141 gatgtacttg gaatttcagt acagttttga aatgtgttta aaatgtatta aaaaagaaca
4201 gcccagccac tataagtacc atgaaaagca ttatgcaaat gctgctatat ttgctgacag
4261 caaaaaccaa aaaaccatat gccaacaggc tgttgatact gttttagcta aaaagcgggt
4321 tgatagccta caattaacta gagaacaaat gttaacaaac agatttaatg atcttttgga
4381 taggatggat ataatgtttg gttctacagg ctctgctgac atagaagaat ggatggctgg
4441 agttgcttgg ctacactgtt tgttgcccaa aatggattca gtggtgtatg actttttaaa
4501 atgcatggtg tacaacattc ctaaaaaaag atactggctg tttaaaggac caattgatag
4561 tggtaaaact acattagcag ctgctttgct tgaattatgt ggggggaaag ctttaaatgt
4621 taatttgccc ttggacaggc tgaactttga gctaggagta gctattgacc agtttttagt
4681 agtttttgag gatgtaaagg gcactggagg ggagtccaga gatttgcctt caggtcaggg
4741 aattaataac ctggacaatt taagggatta tttggatggc agtgttaagg taaacttaga
4801 aaagaaacac ctaaataaaa gaactcaaat atttccccct ggaatagtca ccatgaatga
4861 gtacagtgtg cctaaaacac tgcaggccag atttgtaaaa caaatagatt ttaggcccaa
4921 agattattta aagcattgcc tggaacgcag tgagtttttg ttagaaaaga gaataattca
4981 aagtggcatt gctttgcttc ttatgttaat ttggtacaga cctgtggctg agtttgctca
5041 aagtattcag agcagaattg tggagtggaa agagagattg gacaaagagt ttagtttgtc
5101 agtgtatcaa aaaatgaagt ttaatgtggc tatgggaatt ggagttttag attggctaag
5161 aaacagtgat gatgatgatg aagacagcca ggaaaatgct gataaaaatg aagatggtgg
5221 ggagaagaac atggaagact cagggcatga aacaggcatt gattcacagt cccaaggctc
5281 atttcaggcc cctcagtcct cacagtctgt tcatgatcat aatcagccat accacatttg
5341 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
5401 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
5461 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
5521 ccaaactcat caatgtatct tatcatgtct cgagctgcag atgaatcgta gatactgaaa
5581 aaccccgcaa gttcacttca actgtgcatc gtgcaccatc tcaatttctt tcatttatac
5641 atcgttttgc cttcttttat gtaactatac tcctctaagt ttcaatcttg gccatgtaac
5701 ctctgatcta tagaattttt taaatgacta gaattaatgc ccatcttttt tttggaccta
5761 aattcttcat gaaaatatat tacgagggct tattcagaag ctttggactt cttcgccaga
5821 ggtttggtca agtctccaat caaggttgtc ggcttgtcta ccttgccaga aatttacgaa
5881 aagatggaaa agggtcaaat cgttggtaga tacgttgttg acacttctaa ataagcgaat
5941 ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa
6001 ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg
6061 taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca cacctctacc
6121 ggccggtcga aattccccta ccctatgaac atattccatt ttgtaatttc gtgtcgtttc
6181 tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga gaatcttttt
6241 aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttcggtg gtactgttgg
6301 aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact gcatcttcaa
6361 tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca gcagacaaga
6421 tagtggcgat agggttgacc ttattctttg gcaaatctgg agcagaaccg tggcatggtt
6481 cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca gatggcaaca
6541 aacccaagga acctgggata acggaggctt catcggagat gatatcacca aacatgttgc
6601 tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg gcggcagaat
6661 caatcaattg atgttgaacc ttcaatgtag gaaattcgtt cttgatggtt tcctccacag
6721 tttttctcca taatcttgaa gaggccaaaa cattagcttt atccaaggac caaataggca
6781 atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt tgcacttctg
6841 gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc tttctcttac
6901 caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct ttagcaaatt
6961 gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat ggtcttaagt
7021 tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt ctaacactac
7081 ctgtacccca tttaggacca cccacagcac ctaacaaaac ggcatcagcc ttcttggagg
7141 cttccagcgc ctcatctgga agtgggacac ctgtagcttc gatagcagca ccaccaatta
7201 aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct ttaagaacct
7261 taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct
7321 taggggcaga cattagaatg gtatatcctt gaaatatata tatatattgc tgaaatgtaa
7381 aaggtaagaa aagttagaaa gtaagacgat tgctaaccac ctattggaaa aaacaatagg
7441 tccttaaata atattgtcaa cttcaagtat tgtgatgcaa gcatttagtc atgaacgctt
7501 ctctattcta tatgaaaagc cggttccggc gctctcacct ttcctttttc tcccaatttt
7561 tcagttgaaa aaggtatatg cgtcaggcga cctctgaaat taacaaaaaa tttccagtca
7621 tcgaatttga ttctgtgcga tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat
7681 aatggttgct aagagattcg aactcttgca tcttacgata cctgagtatt cccacagttg
7741 ggggatctcg actctagcta gaggatcaat tcgtaatcat gtcatagctg tttcctgtgt
7801 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
7861 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
7921 tccagtcggg aaacctgtcg tgccagctga taacttcgta taatgtatgc tatacgaagt
7981 tattaggtct gaagaggagt ttacgtccag ccaagctagc ttggctgcag gtcgagcggc
8041 cgcgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag ttatcagctg
8101 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
8161 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
8221 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
8281 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
8341 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
8401 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
8461 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
8521 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
8581 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
8641 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
8701 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
8761 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
8821 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
8881 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
8941 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
9001 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
9061 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
9121 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
9181 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
9241 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
9301 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
9361 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
9421 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
9481 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
9541 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
9601 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
9661 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
9721 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
9781 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
9841 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
9901 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
9961 ctttttcaat at
相关搜索：pGADT7-T纯化费，pGADT7-T Vector